Characterization of Aspergillus oryzae glycoside hydrolase family 43 beta-xylosidase expressed in Escherichia coli

Satoshi Suzuki; Mari Fukuoka; Hikaru Ookuchi; Motoaki Sano; Kenji Ozeki; Emi Nagayoshi; Yukio Takii; Mayumi Matsushita; Sawaki Tada; Ken-Ichi Kusumoto; Yutaka Kashiwagi

doi:10.1016/j.jbiosc.2009.07.018

Characterization of Aspergillus oryzae glycoside hydrolase family 43 beta-xylosidase expressed in Escherichia coli

J Biosci Bioeng. 2010 Feb;109(2):115-7. doi: 10.1016/j.jbiosc.2009.07.018. Epub 2009 Sep 12.

Authors

Satoshi Suzuki¹, Mari Fukuoka, Hikaru Ookuchi, Motoaki Sano, Kenji Ozeki, Emi Nagayoshi, Yukio Takii, Mayumi Matsushita, Sawaki Tada, Ken-Ichi Kusumoto, Yutaka Kashiwagi

Affiliation

¹ National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki, Japan.

PMID: 20129093
DOI: 10.1016/j.jbiosc.2009.07.018

Abstract

This is the first report of glycoside hydrolase family 43 beta-xylosidase from Aspergillus oryzae. To characterize this enzyme, the recombinant enzyme was expressed in Escherichia coli. Unlike known beta-xylosidases from fungal origins, the enzyme did not show substrate ambiguity and was stable at alkaline pH.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aspergillus oryzae / enzymology*
Chromatography, Thin Layer
Enzyme Stability
Escherichia coli / genetics
Escherichia coli / metabolism*
Fungal Proteins / genetics
Fungal Proteins / metabolism*
Hydrogen-Ion Concentration
Phylogeny
Substrate Specificity
Xylosidases / metabolism*

Substances

Fungal Proteins
Xylosidases
exo-1,4-beta-D-xylosidase