Abstract
We evaluated the immunomodulatory activity of type-4 resistant starch (RS) in mesenteric lymph nodes (MLNs) using a rat model. Sixteen male Sprague-Dawley rats were fed either a cellulose diet or an RS diet (RSD) for 4 weeks. Serum immunoglobulin A levels, as well as the CD4(+) T cell population and the ratio of CD4(+)/CD8(+) T cells in the MLNs of rats, were significantly elevated by replacing cellulose with RS in the diet. The survival rate of concanavalin A (Con A)-stimulated MLN lymphocytes and interleukin-4 secretion from the Con A-stimulated MLN lymphocytes were significantly increased in rats fed RSD. These results indicate that type-4 RS might ameliorate allergic inflammation in the MLNs of rats through an increased CD4(+) T cell population and enhanced differentiation of MLN lymphocytes into type-2 T cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CD4-CD8 Ratio
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CD4-Positive T-Lymphocytes / drug effects
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CD4-Positive T-Lymphocytes / metabolism
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CD8-Positive T-Lymphocytes / drug effects
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CD8-Positive T-Lymphocytes / metabolism
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Cell Differentiation / drug effects
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Cell Survival / drug effects
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Cellulose
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Concanavalin A
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Dietary Carbohydrates / administration & dosage*
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Hypersensitivity / drug therapy
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Immunoglobulin A / blood*
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Immunologic Factors / pharmacology*
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Immunologic Factors / therapeutic use
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Inflammation / drug therapy*
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Interleukin-4 / metabolism
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Lymph Nodes / drug effects*
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Lymph Nodes / immunology
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Lymphocytes / drug effects*
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Lymphocytes / metabolism
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Male
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Mesentery
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Rats
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Rats, Sprague-Dawley
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Starch / pharmacology*
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Starch / therapeutic use
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Th2 Cells / drug effects
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Th2 Cells / metabolism
Substances
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Dietary Carbohydrates
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Immunoglobulin A
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Immunologic Factors
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Concanavalin A
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Interleukin-4
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Cellulose
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Starch