Development of a loop-mediated isothermal amplification assay for the detection of Mycobacterium bovis

Vet J. 2011 Mar;187(3):393-6. doi: 10.1016/j.tvjl.2010.01.001. Epub 2010 Feb 7.

Abstract

Bovine tuberculosis caused by Mycobacterium bovis is an important zoonosis. In this study, a simple, rapid method for detecting this organism was developed based on loop-mediated isothermal amplification of the mpt83 gene. The technique will be of value in the clinical and field-based diagnosis of M. bovis and can differentiate it from other bacteria such as Corynebacterium diphtheriae, Streptococcus pneumoniae, β-haemolytic streptococci, Pseudomonas aeruginosa, Yersinia pseudotuberculosis, Staphylococcus aureus, and Klebsiella pneumoniae. The limit of detection was 10 copies/μL and the results were corroborated by PCR. The method was highly specific and more sensitive than PCR.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Animals
  • Cattle
  • Gene Amplification
  • Mycobacterium bovis / genetics
  • Mycobacterium bovis / isolation & purification*
  • Nucleic Acid Amplification Techniques / methods
  • Nucleic Acid Amplification Techniques / standards
  • Nucleic Acid Amplification Techniques / veterinary*
  • Polymerase Chain Reaction / veterinary
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Sputum / microbiology
  • Tuberculosis, Bovine / diagnosis*