Simultaneous genotyping of microsatellite variations in HMOX1 and UGT1A1 genes using multicolored capillary electrophoresis

Alena Jiraskova; Martin Lenicek; Libor Vitek

doi:10.1016/j.clinbiochem.2010.01.006

Simultaneous genotyping of microsatellite variations in HMOX1 and UGT1A1 genes using multicolored capillary electrophoresis

Clin Biochem. 2010 May;43(7-8):697-9. doi: 10.1016/j.clinbiochem.2010.01.006. Epub 2010 Feb 10.

Authors

Alena Jiraskova¹, Martin Lenicek, Libor Vitek

Affiliation

¹ Department of Internal Medicine, Charles University in Prague, Czech Republic. [email protected]

PMID: 20152821
DOI: 10.1016/j.clinbiochem.2010.01.006

Abstract

Objectives: Our aim was to establish a reliable, rapid, and inexpensive method for the simultaneous genotyping of the HMOX-1 (heme oxygenase-1) and UGT1A1 (bilirubin UDP-glucuronosyltransferase) gene promoter variations.

Results: The HMOX1 (GT)(n) and UGT1A1 (TA)(n) gene promoter variations were determined by fragment analysis using a single duplex PCR, with different fluorescent dye-labeled primers; followed by multicolored capillary electrophoresis.

Conclusion: This novel method provides simultaneous genotyping of key tandem repeat variations in the HMOX1 and UGT1A1 promoters.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrophoresis, Capillary / methods*
Genotype
Glucuronosyltransferase / genetics*
Heme Oxygenase-1 / genetics*
Humans
Microsatellite Repeats / genetics*
Polymorphism, Single Nucleotide / genetics
Reproducibility of Results

Substances

Heme Oxygenase-1
UGT1A1 enzyme
Glucuronosyltransferase