Comparison of traditional phenotypic identification methods with partial 5' 16S rRNA gene sequencing for species-level identification of nonfermenting Gram-negative bacilli

J Clin Microbiol. 2010 Apr;48(4):1442-4. doi: 10.1128/JCM.00169-10. Epub 2010 Feb 17.

Abstract

Correct identification of nonfermenting Gram-negative bacilli (NFB) is crucial for patient management. We compared phenotypic identifications of 96 clinical NFB isolates with identifications obtained by 5' 16S rRNA gene sequencing. Sequencing identified 88 isolates (91.7%) with >99% similarity to a sequence from the assigned species; 61.5% of sequencing results were concordant with phenotypic results, indicating the usability of sequencing to identify NFB.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Bacterial Typing Techniques
  • Bacteriological Techniques / methods*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Gram-Negative Bacteria / classification*
  • Gram-Negative Bacteria / isolation & purification*
  • Gram-Negative Bacterial Infections / diagnosis*
  • Gram-Negative Bacterial Infections / microbiology
  • Humans
  • Molecular Sequence Data
  • RNA, Ribosomal, 16S / genetics
  • Sensitivity and Specificity
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S