A flow cytometric assay to quantify in vivo bacterial uptake by alveolar macrophages

Adam M Pitz; Greg A Perry; Heather C Jensen-Smith; Martha J Gentry-Nielsen

doi:10.1016/j.mimet.2010.02.005

A flow cytometric assay to quantify in vivo bacterial uptake by alveolar macrophages

J Microbiol Methods. 2010 May;81(2):194-6. doi: 10.1016/j.mimet.2010.02.005. Epub 2010 Feb 18.

Authors

Adam M Pitz¹, Greg A Perry, Heather C Jensen-Smith, Martha J Gentry-Nielsen

Affiliation

¹ Department of Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178, USA.

PMID: 20171249
DOI: 10.1016/j.mimet.2010.02.005

Abstract

Our laboratory has developed a flow cytometric assay to quantify alveolar macrophage (Mcapital EF, Cyrillic) phagocytosis of bacteria within a live animal. Mcapital EF, Cyrillics collected by bronchoalveolar lavage from rats infected transtracheally with Syto 9-labeled bacteria are fluorescently labeled for identification and analyzed by flow cytometry to quantify their bacterial uptake.

Publication types

Evaluation Study

MeSH terms

Animals
Bacteria / immunology*
Bronchoalveolar Lavage
Flow Cytometry / methods*
Fluorescent Dyes / pharmacology
Lung / immunology
Macrophages, Alveolar / microbiology*
Male
Organic Chemicals / pharmacology
Phagocytosis*
Rats
Rats, Sprague-Dawley
Staining and Labeling

Substances

Fluorescent Dyes
Organic Chemicals
SYTO 9