Abstract
We examined if multilocus sequence typing (MLST), a method for genotyping and species identification of Burkholderia cepacia complex bacteria, could be applied directly to cystic fibrosis sputum. The redesigned nested-PCR MLST format was successfully used to accurately identify strains in 23 sputum samples, of which 8 were culture negative.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Typing Techniques / methods*
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Burkholderia Infections / microbiology*
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Burkholderia cepacia complex / classification*
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Burkholderia cepacia complex / genetics
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Cluster Analysis
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Cystic Fibrosis / complications*
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Genotype
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Humans
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Pneumonia, Bacterial / microbiology*
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Sequence Analysis, DNA
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Sputum / microbiology*