Abstract
Acanthoic acid (AA) is a pimaradiene diterpene isolated from Acanthopanax koreanum. We examined the effect of AA in dextran sulfate sodium (DSS)-induced colitis. AA (100 mg/kg or 300 mg/kg) was administered p.o. daily for 7 days. AA significantly inhibited Disease Activity Index, histological score, and myeloperoxidase activity. Furthermore, AA markedly suppressed the protein expression of TNF-alpha, COX-2, NF-kappaB and chymase as well as the mRNA expression of TNF-alpha and COX-2. These results suggest that AA exerts beneficial effects in experimental colitis, and therefore we propose that this compound may have therapeutic implications for ulcerative colitis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western
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Body Weight / drug effects
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Chymases / biosynthesis
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Colitis, Ulcerative / drug therapy*
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Colitis, Ulcerative / pathology
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Colon / pathology
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Cyclooxygenase 2 / biosynthesis
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Diarrhea / drug therapy
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Diarrhea / etiology
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Diterpenes / chemistry*
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Diterpenes / therapeutic use*
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Eleutherococcus / chemistry*
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Female
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Hemorrhage / drug therapy
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Hemorrhage / etiology
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Mice
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Mice, Inbred BALB C
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NF-kappa B / biosynthesis
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Peroxidase / metabolism
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Plant Roots / chemistry
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RNA, Messenger / biosynthesis
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Reverse Transcriptase Polymerase Chain Reaction
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Tumor Necrosis Factor-alpha / biosynthesis
Substances
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Diterpenes
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NF-kappa B
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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acanthoic acid
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Peroxidase
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Cyclooxygenase 2
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Chymases