Abstract
CHD5 (chromodomain/helicase/DNA-binding protein 5) is a member of the CHD subfamily of chromatin remodeling Swi/Snf proteins, and has been recently identified as a tumor suppressor in a diverse range of human cancers. We report here on the establishment of a hybridoma cell line for producing a monoclonal antibody against CHD5 by the rat medial iliac lymph node method. Immunoblotting analyses indicated that this antibody, MAb 5A10, specifically recognizes endogenous CHD5. In immunostaining using the antibody, a nuclear staining pattern was observed. The monoclonal antibody will be useful in immunoblotting and immunolocalization experiments in a variety of cells and tissues, as well as in further studies of the biological function and cellular dynamics of this protein.
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology*
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Antibodies, Monoclonal / isolation & purification
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Blotting, Western
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DNA Helicases / genetics
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DNA Helicases / immunology*
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Embryo, Mammalian / cytology
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Embryo, Mammalian / immunology
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Enzyme-Linked Immunosorbent Assay
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Female
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Fibroblasts / cytology
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Fibroblasts / immunology
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Fibroblasts / metabolism
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Fluorescent Antibody Technique
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HeLa Cells
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Humans
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Hybridomas / metabolism
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Ileum / immunology
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Immunization
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Immunoenzyme Techniques
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Lymph Nodes / cytology
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Lymph Nodes / immunology
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Mice
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / immunology*
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Rats
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Rats, Inbred WKY
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology*
Substances
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Antibodies, Monoclonal
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Nerve Tissue Proteins
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Recombinant Proteins
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DNA Helicases
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CHD5 protein, human
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CHD5 protein, rat