Flow cytometry vs cytomorphology for the detection of hematologic malignancy in body cavity fluids

Leuk Res. 2010 Aug;34(8):1027-34. doi: 10.1016/j.leukres.2010.02.008. Epub 2010 Mar 5.

Abstract

Flow cytometry and cytomorphology results on 92 body cavity fluids [61 effusions and 31 bronchoalveolar lavage fluids (BALF)] from hematologic malignancy were compared with retrospective clinical outcome. We observed double true positive/negative results in 67 cases (73%), and double false negative results in 2 cases (2%). Immunophenotyping accounted for true positive/negative results in 22 out of 23 mismatched cases (25%), and retained significantly higher accuracy than that of cytomorphology especially in effusions and differentiated lymphoma. In BALF analysis, immunophenotyping and cytomorphology sensitivity was 75% and 0%, respectively. Flow cytometry retains the highest accuracy in detecting neoplastic cells in body cavity fluids.

MeSH terms

  • Aged
  • Biomarkers, Tumor*
  • Body Fluids*
  • Bronchoalveolar Lavage Fluid / chemistry
  • Cell Count
  • False Positive Reactions
  • Female
  • Flow Cytometry*
  • Hematologic Neoplasms / diagnosis*
  • Humans
  • Immunophenotyping*
  • Longitudinal Studies
  • Male
  • Middle Aged
  • Prognosis
  • Retrospective Studies

Substances

  • Biomarkers, Tumor