Effect of NlpE overproduction on multidrug resistance in Escherichia coli

Antimicrob Agents Chemother. 2010 May;54(5):2239-43. doi: 10.1128/AAC.01677-09. Epub 2010 Mar 8.

Abstract

NlpE, an outer membrane lipoprotein, functions during envelope stress responses in Gram-negative bacteria. In this study, we report that overproduction of NlpE increases multidrug and copper resistance through activation of the genes encoding the AcrD and MdtABC multidrug efflux pumps in Escherichia coli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Outer Membrane Proteins / genetics*
  • Copper / pharmacology
  • Drug Resistance, Bacterial / genetics
  • Drug Resistance, Multiple / genetics*
  • Escherichia coli / drug effects*
  • Escherichia coli / genetics*
  • Escherichia coli Proteins / genetics*
  • Gene Expression Regulation, Bacterial / physiology
  • Lipoproteins / genetics*
  • Membrane Transport Proteins / genetics
  • Microbial Sensitivity Tests
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Anti-Bacterial Agents
  • Bacterial Outer Membrane Proteins
  • Escherichia coli Proteins
  • Lipoproteins
  • Membrane Transport Proteins
  • NlpE protein, E coli
  • Copper