Abstract
Two cell-penetrating peptides, a Pro-rich peptide derivative, acetyl-(Val-Arg-Leu-Pro-Pro-Pro)(3)-Gly-Cys amide, and an octaarginine derivative, acetyl-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Cys amide, were prepared by the solid phase method. Each peptide was coupled to the heterobifunctional cross-linking reagent, 6-maleimidohexanoic acid N-hydroxysuccinimide ester, and then conjugated to the Adenovirus vector containing luciferase gene. Peptide-modified Ad, as compared with wild-type Ad, exhibited excellent luciferase activity in B16BL6 cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenoviridae / chemistry
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Adenoviridae / genetics
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Adenoviridae / metabolism*
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Amides / pharmacokinetics
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Amino Acid Sequence
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Animals
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Cell Line, Tumor
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Cell Membrane Permeability
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Coxsackie and Adenovirus Receptor-Like Membrane Protein
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Cross-Linking Reagents
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Genes, Reporter
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Genetic Therapy / methods
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Genetic Vectors / administration & dosage*
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Humans
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Mice
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Oligopeptides / chemical synthesis
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Oligopeptides / chemistry
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Oligopeptides / pharmacokinetics*
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Peptides / chemical synthesis
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Peptides / chemistry
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Peptides / pharmacokinetics*
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Proline / analogs & derivatives*
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Proline / pharmacokinetics
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Receptors, Virus / metabolism
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Transduction, Genetic
Substances
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Amides
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CLMP protein, human
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CLMP protein, mouse
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Coxsackie and Adenovirus Receptor-Like Membrane Protein
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Cross-Linking Reagents
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Oligopeptides
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Peptides
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Receptors, Virus
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octaarginine
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Proline