A new preclinical femoral head osteonecrosis model in sheep

Arch Orthop Trauma Surg. 2011 Jan;131(1):5-9. doi: 10.1007/s00402-010-1084-5. Epub 2010 Mar 9.

Abstract

Introduction: Animal models have been used as insight into the pathogenesis of osteonecrosis, even though most have failed to reproduce all stages of human disease, limiting progression in experimental treatment modalities. A new surgically induced animal model of femoral head osteonecrosis in sheep is presented.

Method: Osteonecrosis was achieved using an improved method of intracephalic cryogenic lesion by means of a cryoprobe and vascular ligation.

Results: Histologic findings at 6 and 12 weeks showed progression to advanced stage osteonecrosis. MRI, the gold standard in diagnosis and follow-up in human osteonecrosis showed concordant results with histology.

Conclusion: Our model can therefore be used as a preclinical femoral head osteonecrosis model in an easily accessible animal to assess biological treatments with MRI.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cold Temperature / adverse effects
  • Disease Models, Animal*
  • Disease Progression
  • Femur Head Necrosis* / pathology
  • Freezing
  • Magnetic Resonance Imaging
  • Sheep