Abstract
Surface plasmon resonance (SPR) is a highly sensitive method for the detection of molecular interactions. One interacting partner is immobilized on the sensor chip surface while the other is injected across the sensor surface. This chapter focuses on high-affinity immobilization of protein substrates for affinity and kinetic analyses using biotin/streptavidin interaction and GST/anti-GST-antibody interaction.
MeSH terms
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Amino Acid Sequence
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Antibodies, Immobilized / chemistry
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Antibodies, Immobilized / immunology
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Antibodies, Immobilized / metabolism
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Biotin / chemistry
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Biotin / metabolism*
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Biotinylation
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Glutathione Transferase / immunology*
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Glutathione Transferase / metabolism
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Immobilized Proteins / chemistry
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Immobilized Proteins / immunology*
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Immobilized Proteins / metabolism*
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Kinetics
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Molecular Sequence Data
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Peptides / chemistry
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Peptides / metabolism
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Recombinant Fusion Proteins / immunology
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Streptavidin / chemistry
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Streptavidin / metabolism
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Surface Plasmon Resonance / methods*
Substances
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Antibodies, Immobilized
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Immobilized Proteins
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Peptides
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Recombinant Fusion Proteins
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Biotin
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Streptavidin
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Glutathione Transferase