Abstract
Anaplastic plasmacytomas (APCTs) from NFS.V(+) congenic mice and pristane-induced plasmacytic PCTs from BALB/c mice were previously shown to be histologically and molecularly distinct subsets of plasma cell neoplasms (PCNs). Here we extended these comparisons, contrasting primary APCTs and PCTs by gene expression profiling in relation to the expression profiles of normal naïve, germinal centre, and memory B cells and plasma cells. We also sequenced immunoglobulin genes from APCT and APCT-derived cell lines and defined surface phenotypes and chromosomal features of the cell lines by flow cytometry and by spectral karyotyping and fluorescence in situ hybridization. The results indicate that APCTs share many features with normal memory cells and the plasma cell-related neoplasms (PLs) of FASL-deficient mice, suggesting that APCTs and PLs are related and that both derive from memory B cells. Published in 2010 by John Wiley & Sons, Ltd.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, N.I.H., Intramural
MeSH terms
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Animals
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B-Lymphocyte Subsets / immunology*
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Base Sequence
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Cell Survival / physiology
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Chromosome Aberrations
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Gene Expression Profiling / methods
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Immunoglobulin Variable Region / genetics
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Immunologic Memory*
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Murine Acquired Immunodeficiency Syndrome / complications
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Murine Acquired Immunodeficiency Syndrome / immunology*
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Murine Acquired Immunodeficiency Syndrome / metabolism
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Neoplasm Proteins / metabolism
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Neoplasms, Plasma Cell / complications
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Neoplasms, Plasma Cell / immunology*
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Neoplasms, Plasma Cell / metabolism
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Oligonucleotide Array Sequence Analysis / methods
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Plasmacytoma / complications
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Plasmacytoma / immunology*
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Plasmacytoma / metabolism
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Signal Transduction / physiology
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Tumor Cells, Cultured
Substances
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Immunoglobulin Variable Region
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Neoplasm Proteins