Abstract
As an extension of the BSP076 study (see the article 'Collaborative Study for the Standardisation of the Histamine Sensitizing Test in Mice and the CHO Cell-based Assay for the Residual Toxicity Testing of Acellular Pertussis Vaccines (BSP076)', page 51 of this issue of Pharmeuropa Bio & Scientific Notes), it was decided to publish the following experimental method for the temperature-based histamine-sensitisation test, validated at the SSI (Statens Serum Institut, Denmark), as a working basis for the growth of the method in individual laboratories.
MeSH terms
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Animal Use Alternatives
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Animals
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Biological Assay / methods*
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Biological Assay / standards
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Body Temperature* / drug effects
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Calibration
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Dose-Response Relationship, Drug
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Female
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Histamine / administration & dosage
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Histamine / immunology
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Histamine / toxicity*
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Laboratories / standards
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Mice
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Mice, Inbred C57BL
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Pertussis Toxin / analysis*
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Pertussis Toxin / immunology
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Pertussis Vaccine / immunology
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Pertussis Vaccine / standards*
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Pertussis Vaccine / toxicity
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Reference Standards
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Thermography
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Toxicity Tests / methods*
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Toxicity Tests / standards
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Vaccines, Acellular / immunology
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Vaccines, Acellular / standards*
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Vaccines, Acellular / toxicity
Substances
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Pertussis Vaccine
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Vaccines, Acellular
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Histamine
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Pertussis Toxin