Crystallization and preliminary X-ray investigation of recombinant human interleukin 4

J Mol Biol. 1991 Apr 20;218(4):675-8. doi: 10.1016/0022-2836(91)90253-3.

Abstract

Crystals of recombinant human interleukin 4 have been grown from solutions of ammonium sulfate. The crystals are tetragonal, space-group P4(1)2(1)2 or P4(3)2(1)2; the unit cell axes are a = 92.2(1) A and c = 46.4(1) A. The crystals are stable to X-rays for at least three days and diffract beyond 2.8 A resolution. The crystals contain approximately 63% solvent, assuming there is one molecule in the asymmetric unit.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Humans
  • Interleukin-4 / chemistry*
  • Protein Conformation
  • Recombinant Proteins / chemistry
  • X-Ray Diffraction

Substances

  • Recombinant Proteins
  • Interleukin-4