An internal domain of the hepatitis B virus X antigen is necessary for transactivating activity

S E Ritter; T M Whitten; A T Quets; R H Schloemer

doi:10.1016/0042-6822(91)90626-m

An internal domain of the hepatitis B virus X antigen is necessary for transactivating activity

Virology. 1991 Jun;182(2):841-5. doi: 10.1016/0042-6822(91)90626-m.

Authors

S E Ritter¹, T M Whitten, A T Quets, R H Schloemer

Affiliation

¹ Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46223.

PMID: 2024502
DOI: 10.1016/0042-6822(91)90626-m

Abstract

A number of deletion mutants of the hepatitis B viral X antigen had been constructed and assayed for their ability to transactivate heterologous viral regulatory elements. Neither the N-terminal nor the C-terminal amino acid residues were required for transactivating activity. Transactivating activity that resided within amino acid residues 32 to 148 of the X antigen did not show any target DNA sequence or cell line specificity.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Cloning, Molecular
DNA Mutational Analysis
Gene Expression Regulation, Viral
Hepatitis B virus / genetics*
Restriction Mapping
Structure-Activity Relationship
Trans-Activators* / genetics*
Viral Regulatory and Accessory Proteins

Substances

Trans-Activators
Viral Regulatory and Accessory Proteins
hepatitis B virus X protein