Aberrant DNA methylation of imprinted H19 gene in human preimplantation embryos

Shi-Ling Chen; Xiao-Yun Shi; Hai-Yan Zheng; Fang-Rong Wu; Chen Luo

doi:10.1016/j.fertnstert.2010.01.120

Aberrant DNA methylation of imprinted H19 gene in human preimplantation embryos

Fertil Steril. 2010 Nov;94(6):2356-8, 2358.e1. doi: 10.1016/j.fertnstert.2010.01.120. Epub 2010 Mar 19.

Authors

Shi-Ling Chen¹, Xiao-Yun Shi, Hai-Yan Zheng, Fang-Rong Wu, Chen Luo

Affiliation

¹ Center for reproductive Medicine, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China. [email protected]

PMID: 20303482
DOI: 10.1016/j.fertnstert.2010.01.120

Abstract

DNA methylation patterns of the H19 differentially methylated region were successfully determined in 32 of 50 human day 3 embryos produced and discarded after assisted reproductive technology procedures. We found methylation patterns similar to those of somatic cells in 81.3% of embryos and demethylation or hypomethylation patterns in 18.7% of embryos.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blastocyst / metabolism*
Cells, Cultured
DNA Methylation* / physiology
Embryo Culture Techniques
Embryonic Development / genetics
Female
Genomic Imprinting* / physiology
Humans
Pilot Projects
Pregnancy
RNA, Long Noncoding
RNA, Untranslated / genetics*
RNA, Untranslated / metabolism
Reproductive Techniques, Assisted
Sequence Analysis, DNA

Substances

H19 long non-coding RNA
RNA, Long Noncoding
RNA, Untranslated