Cytokine profile in pleural fluid and serum after lung transplantation

Transplant Proc. 2010 Mar;42(2):531-4. doi: 10.1016/j.transproceed.2010.01.033.

Abstract

Background: Lung transplantation is the procedure of choice in several end-stage lung diseases. Despite improvements in surgical techniques and immunosuppression, early postoperative complications occur frequently.

Objective: To evaluate the pleural inflammatory response after surgery.

Patients and methods: Twenty patients aged 18 to 63 years underwent unilateral or bilateral lung transplantation between August 2006 and March 2008. Proinflammatory cytokines interleukin (IL)-1beta, IL-6, and IL-8 and vascular endothelial growth factor in pleural fluid and serum were analyzed. For cytokine evaluation, 20-mL samples of pleural fluid and blood (right, left, or both chest cavities) were obtained at 6 hours after surgery and daily until removal of the chest tube or for a maximum of 10 days. Data were analyzed using analysis of variance followed by the Holm-Sidak test.

Results: All effusions were exudates according to Light's criteria. Pleural fluid cytokine concentrations were highest at 6 hours after surgery. Serum concentrations were lower than those in pleural fluid, and IL-1beta, IL-6, and IL-8 were undetectable at all time points.

Conclusions: There is a peak concentration of inflammatory cytokines in the first 6 hours after transplantation, probably reflecting the effects of surgical manipulation. The decrease observed from postoperative day 1 and thereafter suggests the action of the immunosuppression agents and a temporal reduction in pleural inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Cytokines / analysis*
  • Cytokines / blood
  • Exudates and Transudates / metabolism
  • Female
  • Humans
  • Inflammation / blood
  • Interleukin-1beta / analysis
  • Interleukin-1beta / blood
  • Interleukin-6 / analysis
  • Interleukin-6 / blood
  • Interleukin-8 / analysis
  • Interleukin-8 / blood
  • Liver Diseases / classification
  • Liver Diseases / surgery*
  • Lung Transplantation / physiology*
  • Male
  • Middle Aged
  • Pleural Effusion / metabolism
  • Postoperative Complications / epidemiology
  • Retrospective Studies
  • Vascular Endothelial Growth Factor A / analysis
  • Vascular Endothelial Growth Factor A / blood
  • Young Adult

Substances

  • Cytokines
  • Interleukin-1beta
  • Interleukin-6
  • Interleukin-8
  • Vascular Endothelial Growth Factor A