Crystal structure of the C-terminal domain of the Salmonella type III secretion system export apparatus protein InvA

Protein Sci. 2010 May;19(5):1091-6. doi: 10.1002/pro.382.

Abstract

InvA is a prominent inner-membrane component of the Salmonella type III secretion system (T3SS) apparatus, which is responsible for regulating virulence protein export in pathogenic bacteria. InvA is made up of an N-terminal integral membrane domain and a C-terminal cytoplasmic domain that is proposed to form part of a docking platform for the soluble export apparatus proteins notably the T3SS ATPase InvC. Here, we report the novel crystal structure of the C-terminal domain of Salmonella InvA which shows a compact structure composed of four subdomains. The overall structure is unique although the first and second subdomains exhibit structural similarity to the peripheral stalk of the A/V-type ATPase and a ring building motif found in other T3SS proteins respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Crystallography, X-Ray
  • Escherichia coli / genetics
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Salmonella / chemistry*
  • Salmonella / genetics
  • Salmonella / metabolism
  • Sequence Alignment

Substances

  • Bacterial Proteins
  • Recombinant Proteins
  • invA protein, Bacteria