Bioorthogonal turn-on probes for imaging small molecules inside living cells

Angew Chem Int Ed Engl. 2010 Apr 6;49(16):2869-72. doi: 10.1002/anie.200906120. Epub 2010 Mar 19.

Abstract

Glowing tags: a series of activatable ("turn-on") tetrazine-conjugated fluorescent probes was developed, which react rapidly in an inverse-electron-demand [4+2] cycloaddition with strained dienophiles such as trans-cyclooctene, thereby strongly increasing the fluorescence intensity. The novel turn-on probes were applied for intracellular live-cell imaging of a microtubuli-binding trans-cyclooctene modified taxol.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Fluorescent Dyes / chemistry*
  • Microscopy, Confocal
  • Molecular Imaging / methods*
  • Photochemical Processes

Substances

  • Fluorescent Dyes