Abstract
Pertactin, an outer membrane protein of Bordetella pertussis, was purified to apparent homogeneity. The purified pertactin was first extracted from the B. pertussis cells (strain 165) by heating for 1 h at 60 degrees C, followed by DEAE-Sepharose and Affi-Gel Blue chromatography. The purified pertactin migrated as a single band of 69-kDa and a pl of 5.9 and retains a high immunogenicity. The rabbit anti- pertactin antisera shows high specificity in ELISA. The purified pertactin is a potential candidate as immunogen for preparation of diagnostic reagents and as a vaccine component.
MeSH terms
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Antibodies, Bacterial / immunology
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Antigens, Bacterial / immunology
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Bacterial Outer Membrane Proteins / chemistry
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Bacterial Outer Membrane Proteins / immunology*
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Bacterial Outer Membrane Proteins / isolation & purification*
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Blotting, Western
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Bordetella pertussis / metabolism*
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Chromatography, Affinity
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Electrophoresis, Polyacrylamide Gel
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Enzyme-Linked Immunosorbent Assay
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Molecular Weight
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Pertussis Vaccine / immunology
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Virulence Factors, Bordetella / chemistry
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Virulence Factors, Bordetella / immunology*
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Virulence Factors, Bordetella / isolation & purification*
Substances
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Antibodies, Bacterial
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Antigens, Bacterial
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Bacterial Outer Membrane Proteins
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Pertussis Vaccine
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Virulence Factors, Bordetella
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pertactin