Laboratory host systems for extraneous agent testing in avian live virus vaccines: problems encountered

Biologicals. 2010 May;38(3):350-1. doi: 10.1016/j.biologicals.2010.01.005. Epub 2010 Mar 23.

Abstract

The regulation for batch testing of avian live viral vaccines for extraneous agents was modified recently in order to reduce animal use, and routine batch testing had to be adapted to this new regulation. As a result, however, some tests have become more complicated and time-consuming. Due to systematic technical problems, the new methods required could only be applied to a small proportion of the batches produced. As a consequence, the majority of batches are still tested in animals.

MeSH terms

  • Animals
  • Cell Line
  • Chick Embryo
  • Chickens
  • Chlorocebus aethiops
  • Clinical Laboratory Techniques / standards
  • Drug Contamination / prevention & control*
  • Poultry Diseases / immunology
  • Poultry Diseases / prevention & control
  • Poultry Diseases / virology
  • Reproducibility of Results
  • Vero Cells
  • Viral Vaccines / analysis*
  • Viral Vaccines / immunology
  • Viral Vaccines / standards*
  • Viruses / immunology

Substances

  • Viral Vaccines