Objective: To investigate the effect of celecoxib on the cell cycle and apoptosis in human tongue squamous cell carcinoma (HTSCC) cell line Tca8113.
Methods: Tca8113 cell line was cultured in the presence of different concentrations of celecoxib. MTT assay was used to measure cell survival rate, and flow cytometry performed to analyze the cell cycle distribution. Annexin V-FITC/PI staining was used to detect the early changes of apoptosis, and transmission electron microscope employed to observe the ultrastructural changes of the apoptotic cells.
Results: Celecoxib inhibited the proliferation of Tca8113 cells in a dose-dependent manner, the effect of which was mediated by inducing cell cycle arrest mainly in G1/S phase. Flow cytometry and ultrastructural observation demonstrated an early to late stage changes of the apoptotic cells exposed to increased concentrations of celecoxib.
Conclusion: Celecoxib dose-dependently inhibits the proliferation of Tca8113 cells by causing cell cycle arrest and inducing apoptosis.