The purpose of this study was to evaluate collaboration between traditional healers (TH) and registered nurses (RN) in the care of people living with HIV (PLHIV) in the health district N'Gourma Fada, Burkina Faso. A survey was conducted among 26 RN, 33 TH working in the health district and 96 PLHIV under treatment at the Fada N'Gourma Regional Hospital. Survey data showed that only 9.1% had sound knowledge about HIV/AIDS and 18.2% about prevention methods. Conversely 84.8% had a good knowledge about clinical manifestations. Among TH, 84.8% claimed to refer patients to health facilities that provided no support for HIV/AIDS, 51.1% called for establishment of a framework of cooperation and 21.2% asked for reciprocity. Among nurses, 85.2% did not consider TH as part of the health community fighting against HIV/AIDS. Proposals focused on association of TH, frameworks of cooperation, and coordination of TH activities in the fight against HIV/AIDS.