Molecular characterization of toxin A-negative, toxin B-positive variant strains of Clostridium difficile isolated in Korea

Sue Jung Kim; Heejung Kim; Younghee Seo; Dongeun Yong; Seok Hoon Jeong; Yunsop Chong; Kyungwon Lee

doi:10.1016/j.diagmicrobio.2010.01.007

Molecular characterization of toxin A-negative, toxin B-positive variant strains of Clostridium difficile isolated in Korea

Diagn Microbiol Infect Dis. 2010 Jun;67(2):198-201. doi: 10.1016/j.diagmicrobio.2010.01.007. Epub 2010 Mar 24.

Authors

Sue Jung Kim¹, Heejung Kim, Younghee Seo, Dongeun Yong, Seok Hoon Jeong, Yunsop Chong, Kyungwon Lee

Affiliation

¹ Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 120-752, Korea.

PMID: 20338708
DOI: 10.1016/j.diagmicrobio.2010.01.007

Abstract

A(-)B(+)Clostridium difficile strains are prevalent in Korea. We performed pulsed-field gel electrophoresis (PFGE), polymerase chain reaction ribotyping, and toxinotyping in 82 A(-)B(+) clinical isolates in Korea. PFGE showed highest discriminatory capability among the 3 methods. By PFGE, persistence of a clone was found, suggesting this clone has adapted to the hospital environment.

MeSH terms

Bacterial Proteins / genetics*
Bacterial Toxins / genetics*
Bacterial Typing Techniques
Clostridioides difficile / classification*
Clostridioides difficile / genetics
Clostridioides difficile / isolation & purification*
Cluster Analysis
DNA Fingerprinting
Electrophoresis, Gel, Pulsed-Field
Enterocolitis, Pseudomembranous / microbiology*
Enterotoxins / deficiency*
Genotype
Hospitals
Humans
Korea
Molecular Epidemiology
Ribotyping

Substances

Bacterial Proteins
Bacterial Toxins
Enterotoxins
tcdA protein, Clostridium difficile
toxB protein, Clostridium difficile