Objective: To investigate the extraction method of polyglycosides from Tripterygium wilfordii.
Method: The extraction method of pressurized liquid extraction was employed and chromogenic colorimetric technique were used for quantitative analysis. Based on the single-factor experiment, according to the center combination design this paper used three factors and three levels of response surface methodology for process optimization.
Result: The optimized conditions were as follows: ratio of solid to liquid was 1:9.5, at the temperature of 115 degrees C for 80 minutes, the actual extract ratio and purity of polyglycosides obtained were 0.21% and 0.52%, respectively.
Conclusion: The method of pressurized liquid extraction has obvious advantages over conventional reflux extracting.