Abstract
A shortened protocol for two peptide nucleic acid fluorescence in situ hybridization (PNA FISH) assays for the detection of Gram-negative bacilli from positive blood cultures was evaluated in a multicenter trial. There was 100% concordance between the two protocols for each assay (368 of 368 and 370 of 370 results) and 99.7% (367 of 368 and 369 of 370 results) agreement with routine laboratory techniques.
Publication types
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Evaluation Study
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Multicenter Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriological Techniques / methods*
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Blood / microbiology*
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Gram-Negative Bacteria / classification
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Gram-Negative Bacteria / isolation & purification*
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Gram-Negative Bacterial Infections / diagnosis*
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Gram-Negative Bacterial Infections / microbiology
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Humans
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In Situ Hybridization, Fluorescence / methods*
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Peptide Nucleic Acids*
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Sensitivity and Specificity