Abstract
Phosphatidylinositol 3-phosphate (PtdIns(3)P) is a phospholipid residing on early endosomes, where it is proposed to be involved in endosomal fusion. We synthesized membrane-permeant derivatives of PtdIns(3)P, including a caged version that is to our knowledge the first photoactivatable phosphoinositide derivative developed so far. In living cells, photoactivation of caged PtdIns(3)P induced rapid endosomal fusion in an EEA1-dependent fashion, thus providing in vivo evidence that PtdIns(3)P is a sufficient signal for driving this process.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Fusion*
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Cell Membrane Permeability*
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Endosomes / ultrastructure*
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Phosphatidylinositol Phosphates / metabolism*
Substances
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Phosphatidylinositol Phosphates
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phosphatidylinositol 3-phosphate
Associated data
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PubChem-Substance/90944392
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PubChem-Substance/90944393
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PubChem-Substance/90944394
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PubChem-Substance/90944395
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PubChem-Substance/90944396
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PubChem-Substance/90944397
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PubChem-Substance/90944398
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PubChem-Substance/90944399
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PubChem-Substance/90944400
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PubChem-Substance/90944401
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PubChem-Substance/90944402
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PubChem-Substance/90944403
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PubChem-Substance/90944404
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PubChem-Substance/90944405
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PubChem-Substance/90944406
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PubChem-Substance/90944407
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PubChem-Substance/90944408
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PubChem-Substance/90944409
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PubChem-Substance/90944410
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PubChem-Substance/90944411
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PubChem-Substance/90944412
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PubChem-Substance/90944413
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PubChem-Substance/90944414
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PubChem-Substance/90944415
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PubChem-Substance/90944416
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PubChem-Substance/90944417