Development of a rapid, cost-effective TaqMan Real-Time PCR Assay for identification and differentiation of Coccidioides immitis and Coccidioides posadasii

Med Mycol. 2010 May;48(3):466-9. doi: 10.3109/13693780903218990.

Abstract

Coccidioidomycosis is an infection caused by Coccidioides immitis or C. posadasii. We developed a TaqMan real-time PCR assay that rapidly and accurately differentiates the species. This assay can be used as a tool to improve disease surveillance, increase understanding of the natural history of the infection, and assist in clinical differentiation studies.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Coccidioides / classification*
  • Coccidioides / genetics
  • Coccidioides / isolation & purification*
  • Coccidioidomycosis / diagnosis*
  • Coccidioidomycosis / microbiology
  • Humans
  • Polymerase Chain Reaction / economics
  • Polymerase Chain Reaction / methods*
  • Time Factors