Monitoring the efficacy of antimicrobial photodynamic therapy in a murine model of cutaneous leishmaniasis using L. major expressing GFP

J Biophotonics. 2010 Jun;3(5-6):328-35. doi: 10.1002/jbio.201000008.

Abstract

A murine model of cutaneous leishmaniasis with green fluorescent protein positive (GFP+) L. major enables the monitoring of parasitic load via measurements of GFP fluorescence intensity, allowing for a faster and more efficient way of monitoring the clinical outcome of photodynamic therapy (PDT). This model may provide new insights on the phototoxic aspects in PDT. Although PDT regimens may be somewhat different in humans, it is expected that the developed model will facilitate the optimization and clinical translation of PDT as a therapy for cutaneous leishmaniasis and the eventual development of topical PDT treatments for other granulomatous infections.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Calibration
  • Disease Models, Animal
  • Ear Diseases / drug therapy
  • Ear Diseases / parasitology
  • Female
  • Fluorescence
  • Green Fluorescent Proteins
  • Leishmania major / drug effects*
  • Leishmania major / metabolism
  • Leishmaniasis, Cutaneous / drug therapy*
  • Leishmaniasis, Cutaneous / parasitology
  • Mice
  • Mice, Inbred BALB C
  • Phenothiazines / pharmacology
  • Phenothiazines / therapeutic use*
  • Photochemotherapy / methods*
  • Photosensitizing Agents / pharmacology
  • Photosensitizing Agents / therapeutic use*
  • Reproducibility of Results
  • Transfection
  • Treatment Outcome

Substances

  • 3,7-bis(N,N-dibutylamino)phenothiazinium bromide
  • Phenothiazines
  • Photosensitizing Agents
  • Green Fluorescent Proteins