A case of the localized laryngeal amyloidosis was reported. The patient was a 51-year-old female who complained of hoarseness for about a year. Pathological study on the biopsy specimen taken from both the subglottic area under laryngeal microsurgery showed the deposit of amyloid. Laryngeal microsurgery showed the deposit of amyloid at bilateral subglottic area. Electron microscopic study disclosed a typical amyloid fibrillar structure. Amyloid protein, in this case, was confirmed as protein A lambda appeared through the potassium permanganate treatment and immunoperoxidase technique (ABC method).