Chronic constriction injury reduces cannabinoid receptor 1 activity in the rostral anterior cingulate cortex of mice

Brain Res. 2010 Jun 21:1339:18-25. doi: 10.1016/j.brainres.2010.03.105. Epub 2010 Apr 7.

Abstract

The present studies examined the effect of chronic neuropathic pain on cannabinoid receptor density and receptor-mediated G-protein activity within supraspinal brain areas involved in pain processing and modulation in mice. Chronic constriction injury (CCI) produced a significant decrease in WIN 55,212-2-stimulated [(35)S]GTPgammaS binding in membranes prepared from the rostral anterior cingulate cortex (rACC) of CCI mice when compared to sham-operated controls. Saturation binding with [(3)H]SR 141716A in membranes of the rACC showed no significant differences in binding between CCI and sham mice. Analysis of levels of the endocannabinoids anandamide (AEA) or 2-arachidonoylglycerol (2-AG) in the rACC following CCI showed no significant differences between CCI and sham mice. These data suggest that CCI produced desensitization of the cannabinoid 1 receptor in the rACC in the absence of an overall decrease in cannabinoid 1 receptor density or change in levels of AEA or 2-AG. These data are the first to show alterations in cannabinoid receptor function in the rostral anterior cingulate cortex in response to a model of neuropathic pain.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analgesics
  • Animals
  • Arachidonic Acids / metabolism
  • Benzoxazines / metabolism
  • Cannabinoid Receptor Modulators
  • Cell Membrane / metabolism
  • Constriction
  • Disease Models, Animal
  • Endocannabinoids
  • Glycerides
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • Male
  • Mice
  • Mice, Inbred Strains
  • Models, Neurological
  • Morpholines / metabolism
  • Naphthalenes / metabolism
  • Pain / metabolism*
  • Pain / physiopathology
  • Piperidines
  • Polyunsaturated Alkamides / metabolism
  • Prefrontal Cortex / injuries
  • Prefrontal Cortex / metabolism*
  • Prefrontal Cortex / physiopathology
  • Pyrazoles
  • Receptor, Cannabinoid, CB1 / metabolism*
  • Receptor, Cannabinoid, CB1 / physiology*
  • Rimonabant
  • Sulfur Radioisotopes
  • Tritium

Substances

  • Analgesics
  • Arachidonic Acids
  • Benzoxazines
  • Cannabinoid Receptor Modulators
  • Endocannabinoids
  • Glycerides
  • Morpholines
  • Naphthalenes
  • Piperidines
  • Polyunsaturated Alkamides
  • Pyrazoles
  • Receptor, Cannabinoid, CB1
  • Sulfur Radioisotopes
  • Tritium
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • (3R)-((2,3-dihydro-5-methyl-3-((4-morpholinyl)methyl)pyrrolo-(1,2,3-de)-1,4-benzoxazin-6-yl)(1-naphthalenyl))methanone
  • glyceryl 2-arachidonate
  • Rimonabant
  • anandamide