Metagenomic analysis of bronchoalveolar lavage samples from patients with idiopathic interstitial pneumonia and its antagonic relation with Pneumocystis jirovecii colonization

Vicente Friaza; Carmen de la Horra; Mario J Rodríguez-Domínguez; José Martín-Juan; Rafael Cantón; Enrique J Calderón; Rosa Del Campo

doi:10.1016/j.mimet.2010.03.026

Metagenomic analysis of bronchoalveolar lavage samples from patients with idiopathic interstitial pneumonia and its antagonic relation with Pneumocystis jirovecii colonization

J Microbiol Methods. 2010 Jul;82(1):98-101. doi: 10.1016/j.mimet.2010.03.026. Epub 2010 Apr 9.

Authors

Vicente Friaza¹, Carmen de la Horra, Mario J Rodríguez-Domínguez, José Martín-Juan, Rafael Cantón, Enrique J Calderón, Rosa Del Campo

Affiliation

¹ Instituto de Biomedicina de Sevilla, Hospital Universitario, Virgen del Rocío, Seville, Spain.

PMID: 20382190
DOI: 10.1016/j.mimet.2010.03.026

Abstract

Idiopathic interstitial pneumonias are interstitial lung diseases of unknown etiology which prognosis is usually fatal. Microbiota associated to bronchoalveolar lavage from 20 patients with negative bacterial cultures was explored by 16S-rDNA PCR-DGGE, showing a clearly negative relation among the presence of P. jirovecii and bacterial colonization. This is the first report of in vivo antagonistic relation among fungi and bacteria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Bronchoalveolar Lavage Fluid / microbiology*
Female
Humans
Idiopathic Interstitial Pneumonias / microbiology*
Male
Metagenomics*
Middle Aged
Molecular Sequence Data
Pneumocystis carinii / genetics
Pneumocystis carinii / growth & development*
Pneumocystis carinii / isolation & purification*