Crystallization of the plant hormone receptors PYL9/RCAR1, PYL5/RCAR8 and PYR1/RCAR11 in the presence of (+)-abscisic acid

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Apr 1;66(Pt 4):456-9. doi: 10.1107/S1744309110007037. Epub 2010 Mar 31.

Abstract

Abscisic acid (ABA) is a plant hormone that plays key regulatory roles in physiological pathways for the adaptation of vegetative tissues to abiotic stresses such as water stress in addition to events pertaining to plant growth and development. The Arabidopsis ABA receptor proteins PYR/PYLs/RCARs form a START family that contains 14 members which are classified into three subfamilies (I-III). Here, purification, crystallization and X-ray data collection are reported for a member of each of the subfamilies, PYL9/RCAR1 from subfamily I, PYL5/RCAR8 from subfamily II and PYR1/RCAR11 from subfamily III, in the presence of (+)-abscisic acid. The three proteins crystallize in space groups P3(1)21/P3(2)21, P2 and P1, respectively. X-ray intensity data were collected to 1.9-2.6 A resolution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / chemistry*
  • Arabidopsis / chemistry*
  • Arabidopsis Proteins / chemistry*
  • Carrier Proteins / chemistry*
  • Crystallization
  • Crystallography, X-Ray
  • Intracellular Signaling Peptides and Proteins
  • Membrane Transport Proteins / chemistry*

Substances

  • Arabidopsis Proteins
  • Carrier Proteins
  • Intracellular Signaling Peptides and Proteins
  • Membrane Transport Proteins
  • Pyr1 protein, Arabidopsis
  • RCAR1 protein, Arabidopsis
  • Abscisic Acid