Abstract
2-ketoGlc, which is the C(2)-carbon isostere of GlcNAc, is a novel GlcNAc analogue with a ketone group. The corresponding glycosyltransferase donor substrate, UDP-2-ketoGlc, is necessary for synthesizing 2-ketoGlc-containing molecules and is thus highly important for metabolic polysaccharide remodeling and engineering. We report here the first chemoenzymatic synthesis of UDP-2-ketoGlc using our two-enzyme (NahK and GlmU) system in vitro.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Biocatalysis
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Molecular Structure
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Nucleotidyltransferases / chemistry
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Nucleotidyltransferases / metabolism*
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Phosphotransferases / chemistry
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Phosphotransferases / metabolism*
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Stereoisomerism
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Uridine Diphosphate Glucose / analogs & derivatives*
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Uridine Diphosphate Glucose / chemistry
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Uridine Diphosphate Glucose / metabolism*
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Uridine Diphosphate N-Acetylglucosamine / analogs & derivatives
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Uridine Diphosphate N-Acetylglucosamine / chemistry*
Substances
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Uridine Diphosphate N-Acetylglucosamine
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Phosphotransferases
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Nucleotidyltransferases
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UDPacetylglucosamine pyrophosphorylase
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Uridine Diphosphate Glucose