Variability in C(3)-plant cell-wall biosynthesis in a high-CO(2) atmosphere by solid-state NMR spectroscopy

J Am Chem Soc. 2010 May 12;132(18):6335-41. doi: 10.1021/ja909796y.

Abstract

We have used a frequency-selective rotational-echo double-resonance (REDOR) solid-state NMR experiment to measure the concentrations of glycine-glycine pairs in proteins (and protein precursors) of intact leaves of plants exposed to both high- and low-CO(2) atomospheres. The results are interpreted in terms of differences in cell-wall biosynthesis between plant species. We illustrate this variability by comparing the assimilation of label in cheatgrass and soybean leaves labeled using (15)N-fertilizer and (13)CO(2) atmospheres. Cheatgrass and soybean are both C(3) plants but differ in their response to a high-CO(2) environment. Based on REDOR results, we determined that cheatgrass (a plant that seems likely to flourish in future low-water, high-CO(2) environments) routes 2% of the assimilated carbon label that remains in the leaf after 1 h in a 600-ppm (13)CO(2) atmosphere to glycine-rich protein (or its precursors), a structural component of cell walls cross-linked to lignins. In contrast, soybean under the same conditions routes none of its assimilated carbon to glycine-rich protein.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bromus / cytology*
  • Bromus / drug effects
  • Bromus / metabolism*
  • Carbon / metabolism
  • Carbon Dioxide / pharmacology*
  • Cell Wall / drug effects*
  • Cell Wall / metabolism*
  • Dose-Response Relationship, Drug
  • Glycine max / drug effects
  • Glycine max / metabolism
  • Magnetic Resonance Spectroscopy
  • Plant Leaves / cytology
  • Plant Leaves / drug effects
  • Plant Leaves / metabolism

Substances

  • Carbon Dioxide
  • Carbon