Abstract
To characterize the strains of Anaplasma phagocytophilum in wild and domestic animals in China, we isolated the organism from rodents and sheep in northeastern China. We isolated 3 strains (2 from rodents and 1 from sick sheep) through propagation in BALB/c mice and then cell culture in HL60 cells. The 3 isolates were identified by Wright-Giemsa staining, immunofluorescence, and electronic microscopy and were characterized by sequence analyses of the 16S rRNA gene, partial citrate synthase gene, major surface protein 4 gene, and heat shock protein gene. The multiple sequences of the 3 isolates were identical to each other but different from all known strains from other countries. The public health and veterinary relevance of the isolates deserves further investigation.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Anaplasma phagocytophilum / cytology
-
Anaplasma phagocytophilum / genetics
-
Anaplasma phagocytophilum / isolation & purification*
-
Animals
-
China / epidemiology
-
Citrate (si)-Synthase / analysis
-
Citrate (si)-Synthase / genetics
-
Cricetinae
-
DNA, Bacterial / genetics
-
DNA, Bacterial / isolation & purification
-
Ehrlichiosis / epidemiology
-
Ehrlichiosis / microbiology
-
Ehrlichiosis / veterinary*
-
HL-60 Cells
-
Heat-Shock Proteins / analysis
-
Heat-Shock Proteins / genetics
-
Humans
-
Membrane Proteins / analysis
-
Membrane Proteins / genetics
-
Mice
-
Mice, Inbred BALB C
-
Molecular Sequence Data
-
Phylogeny
-
RNA, Ribosomal, 16S / analysis
-
RNA, Ribosomal, 16S / genetics
-
Rodent Diseases / epidemiology
-
Rodent Diseases / microbiology*
-
Sequence Analysis, DNA
-
Sheep
-
Sheep Diseases / epidemiology
-
Sheep Diseases / microbiology*
Substances
-
DNA, Bacterial
-
Heat-Shock Proteins
-
Membrane Proteins
-
RNA, Ribosomal, 16S
-
Citrate (si)-Synthase
Associated data
-
GENBANK/GQ412337
-
GENBANK/GQ412338
-
GENBANK/GQ412339
-
GENBANK/GQ412340
-
GENBANK/GQ412341
-
GENBANK/GQ412342
-
GENBANK/GQ412343
-
GENBANK/GQ412344
-
GENBANK/GQ412345
-
GENBANK/GQ412346
-
GENBANK/GQ412347
-
GENBANK/GQ412348