chinmo is a functional effector of the JAK/STAT pathway that regulates eye development, tumor formation, and stem cell self-renewal in Drosophila

Dev Cell. 2010 Apr 20;18(4):556-68. doi: 10.1016/j.devcel.2010.02.006.

Abstract

The Drosophila STAT transcription factor Stat92E regulates diverse functions, including organ development and stem cell self-renewal. However, the Stat92E functional effectors that mediate these processes are largely unknown. Here we show that chinmo is a cell-autonomous, downstream mediator of Stat92E that shares numerous functions with this protein. Loss of either gene results in malformed eyes and head capsules due to defects in eye progenitor cells. Hyperactivation of Stat92E or misexpression of Chinmo results in blood cell tumors. Both proteins are expressed in germline (GSCs) and cyst stem cells (CySCs) in the testis. While Stat92E is required for the self-renewal of both populations, chinmo is only required in CySCs, indicating that Stat92E regulates self-renewal in different stem cells through independent effectors. Like hyperactivated Stat92E, Chinmo misexpression in CySCs is sufficient to maintain GSCs nonautonomously. Chinmo is therefore a key effector of JAK/STAT signaling in a variety of developmental and pathological contexts.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drosophila Proteins / metabolism
  • Drosophila Proteins / physiology*
  • Drosophila melanogaster
  • Gene Expression Regulation, Developmental*
  • Janus Kinase 1 / metabolism*
  • Male
  • Microscopy, Fluorescence / methods
  • Models, Biological
  • Models, Genetic
  • Nerve Tissue Proteins / metabolism
  • Nerve Tissue Proteins / physiology*
  • Phenotype
  • Photoreceptor Cells, Invertebrate / physiology*
  • STAT Transcription Factors / metabolism*
  • Signal Transduction
  • Stem Cells / cytology*
  • Testis / metabolism

Substances

  • Chinmo protein, Drosophila
  • Drosophila Proteins
  • Nerve Tissue Proteins
  • STAT Transcription Factors
  • Janus Kinase 1