Recombinant Mycobacterium bovis BCG expressing chimaeric protein of Ag85B and ESAT-6 enhances immunostimulatory activity of human macrophages

Ying Xu; Wei Liu; Hongbo Shen; Jingran Yan; Enzhuo Yang; Honghai Wang

doi:10.1016/j.micinf.2010.04.002

Recombinant Mycobacterium bovis BCG expressing chimaeric protein of Ag85B and ESAT-6 enhances immunostimulatory activity of human macrophages

Microbes Infect. 2010 Aug;12(8-9):683-9. doi: 10.1016/j.micinf.2010.04.002. Epub 2010 Apr 22.

Authors

Ying Xu¹, Wei Liu, Hongbo Shen, Jingran Yan, Enzhuo Yang, Honghai Wang

Affiliation

¹ State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, China.

PMID: 20417300
DOI: 10.1016/j.micinf.2010.04.002

Abstract

Recombinant BCG strain that secretes the chimaeric protein of Ag85B and ESAT-6 has been demonstrated to augment Th1 immune response in C57BL/6 mice. In this paper, we studied the immunostimulatory activity of the recombinant BCG strains in vitro and found out that rBCG-A(N)-E-A(C) activated THP-1 cells and induced higher expression levels of CD86, CD80, CD40 and HLA-DR, especially increased the ratio of CD86/CD80. Likewise, rBCG-A(N)-E-A(C) infection was able to stimulate an increase in TNF-alpha production of macrophages. Moreover, rBCG-A(N)-E-A(C) up-regulated the expression of EFHD2, ACTB and ACTG1 in the macrophages and improved the ability of antigen presentation and the CD8(+) T-cells immune response. Taken together, this rBCG-A(N)-E-A(C) strain enhanced the immunostimulatory activity of human macrophages and could be a potential vaccine against Mycobacterium tuberculosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actins / biosynthesis
Acyltransferases / genetics
Acyltransferases / immunology*
Antigens, Bacterial / genetics
Antigens, Bacterial / immunology*
Antigens, CD / biosynthesis
Bacterial Proteins / genetics
Bacterial Proteins / immunology*
Calcium-Binding Proteins / biosynthesis
Cell Line
Electrophoresis, Gel, Two-Dimensional
Gene Expression Profiling
HLA-DR Antigens / biosynthesis
Humans
Macrophages / immunology*
Macrophages / microbiology
Mycobacterium bovis / genetics
Mycobacterium bovis / immunology*
Recombinant Proteins / genetics
Recombinant Proteins / immunology
Tuberculosis Vaccines / genetics
Tuberculosis Vaccines / immunology*
Tumor Necrosis Factor-alpha / biosynthesis

Substances

Actins
Antigens, Bacterial
Antigens, CD
Bacterial Proteins
Calcium-Binding Proteins
EFHD2 protein, human
ESAT-6 protein, Mycobacterium tuberculosis
HLA-DR Antigens
Recombinant Proteins
Tuberculosis Vaccines
Tumor Necrosis Factor-alpha
Acyltransferases
antigen 85B, Mycobacterium tuberculosis