In this review paper, the high-throughput potential of some novel chromatographic modes is surveyed. The modes are Hydrophilic Interaction Liquid Chromatography (HILIC), Supercritical Fluid Chromatography (SFC), and Polar Organic Solvent Chromatography (POSC). Their high throughput potential will be discussed in three domains, i.e. drug discovery, bio-analysis in clinical drug development, and quality control (QC) testing, and is illustrated with some examples.