Change in serum lipid peroxide as an oxidative stress marker and its effects on kidney function after successful kidney transplantation

Transplant Proc. 2010 Apr;42(3):729-32. doi: 10.1016/j.transproceed.2010.02.033.

Abstract

Background: Reactive oxygen species are believed to be responsible for organ injury after reperfusion. We evaluated serial changes in lipid peroxide (LPO) as an oxidative stress marker after kidney transplantation and investigated its effects on graft function.

Methods: Fifty-nine kidney transplant recipients were enrolled between September 2006 and March 2009. The control group consisted of kidney donors (n=40). Serum LPO concentrations were measured by a thiobarbituric acid reaction. The serum creatinine concentration and estimated glomerular filtration rate (eGFR) were used to evaluate graft function. Blood samples were obtained preoperatively, on postoperative day (POD) 5, and at 1 year posttransplantation. The median concentration of LPO on POD 5 was used as a cut-off.

Results: The mean preoperative LPO concentration was greater than the control group. The mean LPO concentration on POD 5 was increased compared with the preoperative level. However, the mean LPO concentration at 1 year was significantly decreased compared with the preoperative day, but greater than the control group. On POD 5, the mean serum creatinine concentration in the low LPO group was lower than that in the high LPO group. The mean eGFR in the low LPO group was significantly higher than that in the high LPO group. There was no difference in mean serum creatinine concentrations and eGFR at 1 year between the groups.

Conclusion: Oxidative stress showed a significant impact on graft function in the immediate posttransplant period.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Biomarkers / blood
  • Chi-Square Distribution
  • Creatinine / blood
  • Female
  • Follow-Up Studies
  • Glomerular Filtration Rate / physiology*
  • Graft Rejection / epidemiology
  • Humans
  • Immunosuppressive Agents / therapeutic use
  • Kidney Function Tests
  • Kidney Transplantation / immunology
  • Kidney Transplantation / physiology*
  • Lipid Peroxides / blood*
  • Living Donors*
  • Male
  • Middle Aged
  • Oxidative Stress / physiology*
  • Reoperation / statistics & numerical data
  • Treatment Outcome

Substances

  • Biomarkers
  • Immunosuppressive Agents
  • Lipid Peroxides
  • Creatinine