Abstract
There are no ideal ways to identify and isolate viable and purified Foxp3(+) regulatory T cells so far. Here we developed a novel procedure for the isolation of highly purified Foxp3(+) cells using flow cytometry. This method relies on an identification and sorting of the lymphoblast cell population identified on a scatter plot using flow cytometry. We confirmed that greater than 98% of the cells sorted using this technique expressed Foxp3 and displayed a potent suppressive activity. This method provides a valuable tool for the study of the T regulatory cell biology and their therapeutic manipulation.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Cell Separation*
-
Female
-
Flow Cytometry*
-
Forkhead Transcription Factors / genetics
-
Forkhead Transcription Factors / metabolism*
-
Gene Knock-In Techniques
-
Green Fluorescent Proteins / genetics
-
Green Fluorescent Proteins / metabolism
-
Interleukin-2 Receptor alpha Subunit / metabolism
-
Mice
-
Mice, Inbred C57BL
-
T-Lymphocytes, Regulatory / cytology*
-
T-Lymphocytes, Regulatory / immunology
Substances
-
Forkhead Transcription Factors
-
Foxp3 protein, mouse
-
Interleukin-2 Receptor alpha Subunit
-
Green Fluorescent Proteins