Replacing vascular corrosion casting by in vivo micro-CT imaging for building 3D cardiovascular models in mice

Mol Imaging Biol. 2011 Feb;13(1):78-86. doi: 10.1007/s11307-010-0335-8.

Abstract

Purpose: The purpose of this study was to investigate if in vivo micro-computed tomography (CT) is a reliable alternative to micro-CT scanning of a vascular corrosion cast. This would allow one to study the early development of cardiovascular diseases.

Procedures: Datasets using both modalities were acquired, segmented, and used to generate a 3D geometrical model from nine mice. As blood pool contrast agent, Fenestra VC-131 was used. Batson's No. 17 was used as casting agent. Computational fluid dynamics simulations were performed on both datasets to quantify the difference in wall shear stress (WSS).

Results: Aortic arch diameters show 30% to 40% difference between the Fenestra VC-131 and the casted dataset. The aortic arch bifurcation angles show less than 20% difference between both datasets. Numerically computed WSS showed a 28% difference between both datasets.

Conclusions: Our results indicate that in vivo micro-CT imaging can provide an excellent alternative for vascular corrosion casting. This enables follow-up studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Vessels / anatomy & histology*
  • Cardiovascular Diseases / pathology
  • Corrosion
  • Mice
  • Models, Anatomic*
  • Tomography, X-Ray Computed / methods*