Abstract
A meat factory commensal bacterium, Acinetobacter calcoaceticus, affected the spatial distribution of Escherichia coli O157:H7 surface colonization. The biovolume of E. coli O157:H7 was 400-fold higher (1.2 x 10(6) microm(3)) in a dynamic cocultured biofilm than in a monoculture (3.0 x 10(3) microm(3)), and E. coli O157:H7 colonized spaces between A. calcoaceticus cell clusters.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acinetobacter calcoaceticus / growth & development*
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Acinetobacter calcoaceticus / ultrastructure
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Biofilms / growth & development*
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Coculture Techniques
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Colony Count, Microbial
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Escherichia coli O157 / genetics
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Escherichia coli O157 / growth & development*
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Escherichia coli O157 / ultrastructure
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Food Contamination
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Food-Processing Industry*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Meat* / microbiology
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Microscopy, Confocal
Substances
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Green Fluorescent Proteins