Purification on poly(U)-sepharose 4B of human breast cancer cell line T-47D DNA polymerases

J Chromatogr. 1991 Feb 22;539(2):511-6. doi: 10.1016/s0021-9673(01)83962-5.

Abstract

The purification of DNA polymerases (RNA-directed DNA polymerases and DNA-directed DNA polymerases) on poly(U)-Sepharose 4B from a breast tumour cell line (T-47D) is reported. The elution of these enzymes was followed in each fraction by activity measurements with the four primer-templates poly(rA)-oligo(dT)12-18, poly(dA) oligo(dT)12-18, poly(rC)-oligo(dG)12-18 and poly(rCm)-oligo (dG)12-18. The control of the polymerase purification by chromatography was performed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis of the pooled active enzymatic fractions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / chemistry*
  • Breast Neoplasms / pathology
  • Cell Line
  • Chromatography, Ion Exchange / instrumentation
  • Chromatography, Ion Exchange / methods*
  • DNA-Directed DNA Polymerase / analysis
  • DNA-Directed DNA Polymerase / isolation & purification*
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Poly U*
  • Sepharose*

Substances

  • Poly U
  • Sepharose
  • DNA-Directed DNA Polymerase