Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O12

Angelina A Parkhomchuk; Nina A Kocharova; Magdalena Białczak-Kokot; Alexander S Shashkov; Alexander O Chizhov; Yuriy A Knirel; Antoni Rozalski

doi:10.1016/j.carres.2010.04.005

Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O12

Carbohydr Res. 2010 Jun 16;345(9):1235-9. doi: 10.1016/j.carres.2010.04.005. Epub 2010 Apr 10.

Authors

Angelina A Parkhomchuk¹, Nina A Kocharova, Magdalena Białczak-Kokot, Alexander S Shashkov, Alexander O Chizhov, Yuriy A Knirel, Antoni Rozalski

Affiliation

¹ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation. [email protected]

PMID: 20471004
DOI: 10.1016/j.carres.2010.04.005

Abstract

The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O12. Its structure was studied by sugar analysis using GLC of the alditol acetates and (S)-2-octyl glycosides, methylation analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy, including 2D (1)H-(1)H COSY, TOCSY, ROESY, (1)H-(13)C HSQC, and HMBC experiments. It was found that the polymer is a neutral heteropolysaccharide and has a branched heptasaccharide repeating unit with the following structure:

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Magnetic Resonance Spectroscopy
Mass Spectrometry
Molecular Sequence Data
O Antigens / chemistry*
O Antigens / isolation & purification
Providencia / chemistry*

Substances

O Antigens