Synthesis of the Cyanine 7 labeled neutrophil-specific agents for noninvasive near infrared fluorescence imaging

Bioorg Med Chem Lett. 2010 Jun 15;20(12):3515-7. doi: 10.1016/j.bmcl.2010.04.136. Epub 2010 May 18.

Abstract

A neutrophil-binding peptide, cinnamoyl-F(D)LF(D)LF (cFLFLF), was labeled with the near infrared (NIR) fluorophore, Cyanine 7 (Cy7). This construct was modified with a polyethylene glycol (PEG, M(W) 3.4 kDa) moiety in order to increase its solubility and bioavailability to circulating neutrophils. A preliminary noninvasive fluorescence imaging of a mouse model of ear inflammation with the fluorescent probe is presented.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzothiazoles* / chemistry
  • Biological Availability
  • Carbocyanines* / chemistry
  • Diagnostic Imaging / methods*
  • Fluorescence
  • Fluorescent Dyes / chemical synthesis*
  • Inflammation / diagnosis
  • Infrared Rays*
  • Mice
  • Neutrophils / metabolism
  • Neutrophils / pathology*
  • Polyethylene Glycols
  • Solubility

Substances

  • Benzothiazoles
  • Carbocyanines
  • Fluorescent Dyes
  • Polyethylene Glycols
  • cyanine dye 7