Development of rapid immunoassays for the detection of ractopamine in swine urine

Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2010 Aug;27(8):1096-103. doi: 10.1080/19440041003754985.

Abstract

The monoclonal antibodies (mAbs) against ractopamine (Rac) were prepared and their properties identified by indirect competitive enzyme-linked immunoabsorbant assay (ELISA). The IC(50) of mAbs was 2.7 ng ml(-1) towards Rac or 9.3 ng ml(-1) towards Rac-glucuronides and no cross-reactivity (CR) towards other competitors except dobutamine (CR: 3.76%). Based on the mAbs, the Rac-kit (kit) and Rac-strip (strip) were developed to detect Rac residues in swine urine. The strip and kit assay could be performed within 5-10 min and 2 h, respectively, allowing the analysis of urine samples without the need for sample clean-up. The detection limits were 1 ng ml(-1) for kit and 3 ng ml(-1) with the unaided eye, and 0.2 ng ml(-1) with the Strip Reader for strip. The correlation coefficients (R(2)) were 0.988 for kit in the range 0-128.0 ng ml(-1), and 0.987 for strip in the range 0-10.8 ng ml(-1). Comparing the gas chromatography-mass spectrometry (GC-MS) with the kit or strip in swine urine spiked with Rac standards, the differences ranged from 1.4% to 4.5% for kit and 1.0% to 4.7% for strip. However, the differences were greater than 54% for the kit and 55% for the strip test for the analysis of urine from swine treated with Rac. The results obtained from GC-MS using hydrolysed urine samples were generally in good agreement with those obtained from strip or kit using non-hydrolysed urine samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenergic beta-Agonists / chemistry
  • Adrenergic beta-Agonists / urine*
  • Animal Feed
  • Animals
  • Antibodies, Monoclonal
  • Antibody Specificity
  • Calibration
  • Cross Reactions
  • Drug Residues / analysis
  • Drug Residues / standards
  • Enzyme-Linked Immunosorbent Assay / instrumentation
  • Enzyme-Linked Immunosorbent Assay / methods
  • Food Additives / analysis
  • Food Additives / chemistry
  • Food Contamination / prevention & control
  • Limit of Detection
  • Meat-Packing Industry / economics
  • Meat-Packing Industry / instrumentation
  • Meat-Packing Industry / methods*
  • Off-Label Use / veterinary
  • Phenethylamines / chemistry
  • Phenethylamines / urine*
  • Reagent Strips
  • Reproducibility of Results
  • Swine / urine*
  • Time Factors
  • Veterinary Drugs / chemistry
  • Veterinary Drugs / urine*

Substances

  • Adrenergic beta-Agonists
  • Antibodies, Monoclonal
  • Food Additives
  • Phenethylamines
  • Reagent Strips
  • Veterinary Drugs
  • ractopamine